Caffeine coingested with carbohydrate on performance recovery in national-level paddlers: a randomized, double-blind, crossover, placebo-controlled trial.

BACKGROUND: Caffeine enhances muscle glycogen resynthesis postexercise; however, the next-day effects on recovery are unknown. The present study aimed to examine the effects of carbohydrate (CHO) supplementation with or without caffeine (CAF) 24-h following exhaustive exercise on time trial performance in elite paddling athletes. METHODS: Nine highly trained male paddlers (21±2 y) completed three experimental trials in a randomized, double-blind, crossover manner. Following an exhaustive exercise session (20-km timed paddle) participants ingested: 1) 0.6 g/kg of carbohydrate (CHO); 2) 0.6 g/kg of carbohydrate with 6 mg/kg of caffeine (CAF+CHO); or 3) placebo (PLA), at four time-points (immediately after, and 2, 6, and 12-h postexercise) in addition to their typical dietary intake. After 24 h, 5 attempts of on-water 500-m paddling time-trial was performed, and the average time was recorded. Blood samples were taken at rest and following both the 20-km and the 5×500 m exercise to determine changes in plasma cortisol, insulin, and glucose. RESULTS: There was a significant main effect of condition (P<0.001), with post-hoc analysis revealing that both CHO conditions (CHO: 98.7±2.8 s, P=0.0003; CAF+CHO: 97.9±2.3 s, P=0.0002) were significantly faster compared to PLA (101.0±3.1 s); however, CAF did not augment time trial performance compared to CHO (P=0.16). There was no significant condition by time interactions for glucose, cortisol, or insulin before and after the 20-km depleting exercise and 500-m time trial. CONCLUSIONS: In elite male paddlers, CHO, independent of caffeine, enhanced time trial performance 24 hours following exhaustive exercise.

High-Intensity Interval vs. Continuous Endurance Training: Preventive Effects on Hormonal Changes and Physiological Adaptations in Prediabetes Patients.

ABSTRACT: Safarimosavi, S, Mohebbi, H, and Rohani, H. High-intensity interval vs. continuous endurance training: Preventive effects on hormonal changes and physiological adaptations in prediabetes patients. J Strength Cond Res 35(3): 731-738, 2021-The aim of this study was to examine the effects of a 12-week high-intensity interval training (HIIT) intervention, or an isocaloric continuous endurance training (CET) intervention on insulin resistance indices and change in irisin and preptin in patients with prediabetes. Thirty-two prediabetic male patients (age = 38.7 ± 4; body mass index = 26.9 ± 1.4 kg·m-2; and V̇o2peak = 2.49 ± 0.22 L·min-1) were randomly assigned into 3 training groups (N = 8). These groups were matched based on the required energy expenditure (EE) for completing each protocol: (a) HIIT (10 × 60 seconds at 90% peak oxygen uptake [V̇o2peak], 1: 1 work to recovery at 50 W), (b) CET at an intensity equivalent to maximal fat oxidation (Fatmax) (CETFAT) (pedaling for a duration that expends an equivalent EE to an HIIT session [E ≈ HIIT]), (c) CET at an intensity equivalent to anaerobic threshold (CETAT) (E ≈ HIIT), and (d) the control group (CON): continued to perform their daily activities. After intervention, blood glucose levels were significantly (p < 0.05) lower in the HIIT group compared with CETAT group. Exercise training improved the insulin resistance index by 35, 28, and 37% in CETFAT, CETAT, and HIIT groups, respectively. Irisin concentrations in the HIIT and CETAT groups was significantly (p < 0.05) decreased compared with the pre-training values. Also, HIIT and CETFAT resulted in significant (p < 0.05) changes in preptin concentration compared with baseline. This study demonstrated that both HIIT and CETFAT protocols had similar effects on the insulin resistance index of prediabetic patients. Also, the intensity and type of exercise were effective factors in changing irisin and preptin concentrations.

The Impact of Moderate-Intensity Continuous or High-Intensity Interval Training on Adipogenesis and Browning of Subcutaneous Adipose Tissue in Obese Male Rats.

This study compares the effect of two types of exercise training, i.e., moderate-intensity continuous training (MICT) or high-intensity interval training (HIIT) on the browning of subcutaneous white adipose tissue (scWAT) in obese male rats. Effects on fat composition, metabolites, and molecular markers of differentiation and energy expenditure were examined. Forty male Wistar rats were assigned to lean (n = 8) or obese (n = 32) groups and fed either a standard chow or high-fat obesogenic diet for 10 weeks. Eight lean and obese rats were then blood and tissue sampled, and the remaining obese animals were randomly allocated into sedentary, MICT, or HIIT (running on a treadmill 5 days/week) groups that were maintained for 12 weeks. Obesity increased plasma glucose and insulin and decreased irisin and FGF-21. In scWAT, this was accompanied with raised protein abundance of markers of adipocyte differentiation, i.e., C/EBP-α, C/EBP-ß, and PPAR-γ, whereas brown fat-related genes, i.e., PRDM-16, AMPK/SIRT1/PGC-1α, were reduced as was UCP1 and markers of fatty acid transport, i.e., CD36 and CPT1. Exercise training increased protein expression of brown fat-related markers, i.e., PRDM-16, AMPK/SIRT1/PGC-1α, and UCP1, together with gene expression of fatty acid transport, i.e., CD36 and CPT1, but decreased markers of adipocyte differentiation, i.e., C/EBP-α, C/EBP-ß, and plasma glucose. The majority of these adaptations were greater with HIIT compared to MICT. Our findings indicate that prolonged exercise training promotes the browning of white adipocytes, possibly through suppression of adipogenesis together with white to beige trans-differentiation and is dependent on the intensity of exercise.

Hyperinsulinaemia and hyperglycaemia promote glucose utilization and storage during low- and high-intensity exercise.

PURPOSE: The effect of hyperglycaemia with and without additional insulin was explored at a low and high intensity of exercise (40% vs 70% VO2peak) on glucose utilization (GUR), carbohydrate oxidation, non-oxidative glucose disposal (NOGD), and muscle glycogen. METHODS: Eight healthy trained males were exercised for 120 min in four trials, twice at 40% VO2peak and twice at 70% VO2peak, while glucose was infused intravenously (40%G; 70%G) at rates to "clamp" blood glucose at 10 mM. On one occasion at each exercise intensity, insulin was also infused at 40 mU/m2/per min (i.e. 40%GI and 70%GI). The glucose and insulin infusion began 30 min prior to exercise and throughout exercise. A muscle biopsy was taken at the end of exercise for glycogen analysis. RESULTS: Hyperglycaemia significantly elevated plasma insulin concentration (p < 0.001), although no difference was observed between the exercise intensities. Insulin infusion during both mild and severe exercise resulted in increased insulin concentrations (p < 0.01) and GUR (p < 0.01) compared with glucose (40%GI by 25.2%; 70%GI by 26.2%), but failed to significantly affect carbohydrate, fat and protein oxidation. NOGD was significantly higher for GI trials at both intensities (p < 0.05) with storage occurring during both lower intensities (62.7 ± 19.6 g 40%GI; 127 ± 20.7 g 40%GI) and 70%GI (29.0 ± 20.0 g). Muscle glycogen concentrations were significantly depleted from rest (p < 0.01) after all four trials. CONCLUSION: Hyperinsulinaemia in the presence of hyperglycaemia during both low- and high-intensity exercise promotes GUR and NOGD, but does not significantly affect substrate oxidation.

High-intensity interval training increase GATA4, CITED4 and c-Kit and decreases C/EBPß in rats after myocardial infarction.

AIM: Myocardial infarction (MI), an important cause of morbidity and mortality, can be followed by left ventricular dysfunction and cardiomyocyte loss. Cardiac repair mechanisms may subsequently improve left ventricular function. Exercise training has been suggested to have cardioprotective effects against MI damage, but detailed knowledge is lacking on the effects of different types and intensities of exercise training on molecular targets of cardiomyocyte regeneration. MAIN METHODS: MI was induced in male Wistar rats by ligating the left anterior descending coronary artery. After MI induction, the rats were randomly assigned to one of five groups: sham operated, and experimental MI followed by no exercise, or low, moderate or high intensity exercise Cardiac function and infarct size were assessed by echocardiography and Evans blue/TTC staining, respectively. The expression of mRNA markers and proteins associated with myocardial regeneration was measured with RT-PCR and western blotting. KEY FINDINGS: Exercise training at different intensities improved cardiac function and levels of stem cell and cardiomyocyte markers, and reduced infarct size. mRNA levels of GATA4, Nkx2.5 and c-Kit and protein expression of Nkx2.5 and c-Kit were significantly increased in all MI-exercise groups. The high-intensity exercise group had greater increases than the low and moderate intensity exercise groups. In the high-intensity exercise group, Sca-1 and CITED4 increased more than in the low-intensity exercise group. C/EBPß mRNA and protein levels decreased after exercise training, with greater reductions in the high-intensity exercise group than the low- or moderate-intensity groups. SIGNIFICANCE: The findings suggest that by targeting cardiogenesis, high-intensity training can exert cardioprotective effects against cardiac dysfunction in an experimental model of MI.

Phonon engineering in proximity enhanced superconductor heterostructures.

In this research, we tailor the phonon density of states (DOS) in thin superconducting films to suppress quasiparticle losses. We examine a model system of a proximity-enhanced three-layered Al/Nb/Al heterostructure and show that the local quantized phonon spectrum of the ultrathin Al cladding layers in the heterostructure has a pronounced effect on the superconducting resonator's quality factors. Instead of a monotonic increase of quality factors with decreasing temperatures, we observe the quality factor reaches a maximum at 1.2 K in 5/50/5 nm Al/Nb/Al microstrip resonators, because of a quantized phonon ladder. The phonon DOS may be engineered to enhance the performance of quantum devices.

Efecto de la masa muscular implicada en los ejercicios de resistencia sobre la presión arterial postejercicio y el doble producto / Effect of involved muscle mass in resistance exercise on post-exercise blood pressure and rate pressure product

La presión arterial y la frecuencia cardíaca (FC) durante el período de recuperación pueden estar influenciadas por el tamaño de la masa muscular activa, y es posible que esta variable también tenga algunos efectos diferentes sobre el doble producto en el postejercicio (RPP). El objetivo del presente estudio fue investigar y comparar la presión arterial sistólica (PAS) y la presión arterial diastólica (PAD), la FC y el RPP durante la recuperación de ejercicio de resistencia con distintas regiones del cuerpo. Doce jóvenes normotensos realizaron al azar 3 ensayos de ejercicio de resistencia con los miembros superiores (MS), los miembros inferiores (MI) y todo el cuerpo (TC), y una sesión de control (CON). Todos los tipos de ejercicio incluyeron 3 series de 10 repeticiones con un 65% de 1RM con 2 min de intervalo de descanso entre series y ejercicios. La PAS, la PAD, la FC, y el RPP se midieron antes y cada 15 min después del ejercicio, hasta 60 min. El lactato sanguíneo (lacS) también se midió antes y en el primer minuto después del ejercicio. Los análisis de varianza (ANOVA) y de Bonferroni post hoc se utilizaron para analizar los datos a p ≤ 0,05. La PAS disminuyó durante los 60 min postejercicio de MS, MI y TC de manera similar, mientras que después del ejercicio la PAD no mostraba ningún cambio. Se observaron aumentos significativos en RPP durante 15 min después del elercicio de MI y 30 min después del de MS y de TC. La concentración lacS aumentó significativamente después del ejercicio de resistencia de MS, de MI y de TC en comparación con los valores de descanso, así como sesiones CON. Los diferentes ejercicios de resistencia de MS, MI y TC provocan una hipotensión post ejericio de similar magnitud y duración y casi producen las mismas respuestas cardiovasculares, a pesar de que en ellos participa una masa muscular diferente

As the blood pressure (BP) and heart rate (HR) response during the recovery period can be influenced by size of activated muscle mass, it is possible that this variable also has some distinct effects on post-exercise rate pressure product (RPP). The aim of the present study was to investigate and compare the systolic (SBP) and diastolic blood pressure (DBP), HR, and RPP responses during recovery to resistance exercise with different body regions. Twelve normotensive young men randomly performed three resistance exercise trials with upper limbs (UL), lower limbs (LL), and whole body (WB), and a control session (CON). All exercise trails included 3 sets of 10 repetitions, with 65% of 1-repetition maximum (1RM) with 2 min rest interval between sets and exercises. SBP, DBP, HR, and RPP were measured before and at each 15 min after exercise until 60 min. Blood lactate (bLAC) was also measured before, and at the 1st minute after the end of exercise. Analysis of variance (ANOVA) and Bonferroni post hoc were used to analyze the data, with a P < .05. The SBP decrease for 60 min after UL, LL, and WB exercise trials was similar, whereas there was no change in post-exercise DBP after the trials. Significant increases were observed in RPP for 15 min after LL, and 30 min after UL and WB. The blood lactate concentration significantly increased after UL, LL and WB resistance exercise when compared to the rest values, as well as in the CON sessions. UL, LL and WB resistance exercise lead to post-exercise hypotension, similar in magnitude and duration, and almost produce the same cardiovascular responses, despite different muscle mass involvement

El efecto de 12 semanas de entrenamiento de resistencia con 2 intensidades diferentes en la expresión de GLUT4 mARN en los músculos sóleo y gastrocnemio en ratones obesos / The effect of 12 weeks endurance training at 2 different intensities on GLUT4 mRNA expression of soleus and gastrocnemius muscles in obese mice

El objetivo de este estudio fue investigar la expresión de mARN de GLUT4 en los músculos sóleo y gastrocnemio en ratones obesos en respuesta al entrenamiento de resistencia. Cuarenta machos C57BL/6 ratones fueron utilizados en este estudio. Ocho ratones (normal base [NB]) sirvieron como no obesos controles no entrenados, y 32 ratones fueron puestos en una dieta alta en grasa (HFD), régimen que siguieron (60% de grasa, kcal) durante 12 semanas. En la semana 16 los ratones obesos fueron distribuidos aleatoriamente en los siguientes grupos de tratamiento (n = 8 cada grupo): base obesos (OB); baja intensidad (LI); alta intensidad (HI); o grupos de control (OC) obesos. LI y HI fueron entrenados durante 5 días/semana durante 12 semanas en una cinta rodante motorizada a 15 m/min en una pendiente del 5% (para LI) y/o en 22 m/min en una pendiente 5% (para HI). Los ratones OCse mantuvieron sedentariamente en la jaula durante el programa de formación. GLUT4 expresión de mRNA se midió en los músculos gastrocnemio y sóleo, utilizando el método en real time-PCR. La expresión de GLUT4 del mARN del músculo sóleo en el grupo LI aumentó aproximadamente ∼2,2 veces, frente a ∼1,6 veces para los gemelos (p ≤ 0,05). Además, la expresión de GLUT4 mARN en los músculos sóleo y gastrocnemio en los grupos LI y HI fue significativamente mayor que en OB y en los grupos OC (p ≤ 0,05). Se puede concluir que cualquier alteración en el equilibrio energético del cuerpo, especialmente por la práctica de ejercicio y/o dieta alta en grasas puede influir en esos mecanismos moleculares y celulares que actúan para establecer una homeostasis estable

The aim of this study was to investigate the expression of GLUT4 mRNA in soleus and gastrocnemius muscles in obese mice in response to endurance training. Forty male C57BL/6 mice were used in this study. Eight mice (Normal Base [NB]) served as non-obese non-trained controls, and 32 mice were put on a high fat diet (HFD) regimen (60% kcal fat) for 12 weeks. At week 16, the obese mice were randomized into the following treatment groups (n = 8 each group): Obese Base [OB]; Low Intensity [LI]; High Intensity [HI]; or Obese Control [OC] groups. LI and HI trained for 5 days/week for 12 weeks on a motorized treadmill at 15 m/min on a 5% slope (for LI), and/or at 22 m/min on a 5% slope (for HI). OC mice were kept sedentarily in the cage during the training program. GLUT4 mRNA expression was measured in gastrocnemius and soleus muscles using a Real Time-PCR method. GLUT4 mRNA expression of soleus muscle in LI group increased about 2.2 fold, against about 1.6 fold for gastrocnemius (p < .05). In addition, GLUT4 mRNA expression of soleus and gastrocnemius muscles in LI and HI groups were significantly higher than OB and OC groups (p < .05). It can be concluded that any disturbance in body energy balance, especially by exercise training and/or high fat diet can influence these molecular and cellular mechanisms that act to establish a stable homeostasis

Effects of exercise at individual anaerobic threshold and maximal fat oxidation intensities on plasma levels of nesfatin-1 and metabolic health biomarkers.

Exercise is recognized as an effective method of weight management and short-term appetite regulation tool. The effect of different exercise intensities on appetite regulation hormones in healthy overweight participants has not been intensively studied. The aim of this study was to examine the influence of exercise at individual anaerobic threshold (IAT) and maximal fat oxidation (Fatmax) intensities on the nesfatin-1 response and metabolic health biomarkers in overweight men. Nine healthy overweight males (age, 23.1 ± 1.1 years) volunteered in this study in a counterbalanced order. Blood samples were obtained before, immediately after, and following the first 45 min of recovery for measuring plasma variables. There was significant decrease in plasma levels of nesfatin-1 and leptin after exercise at the IAT intensity which remained lower than baseline following 45 min of recovery. However, nesfatin-1 and leptin levels did not change significantly in any time courses of Fatmax intensity (P > 0.09). Plasma interleukin-6 (IL-6) concentration increased during exercise in both intensities (P < 0.05), whereas changes in free fatty acids (FFAs) and epinephrine concentrations were significant only at the IAT. In addition, a significant correlation was found among nesfatin-1 levels with insulin (r = 0.39, P < 0.05) and glucose (r = 0.41, P < 0.05) at basal and in response to exercise. These results indicate that IAT has a greater exercise-induced appetite regulation effect compared with Fat(max). Based on these data, the intensity of exercise may have an important role in changes of nesfatin-1, leptin, FFA, and epinephrine concentrations even though this was not the case for IL-6 and insulin resistance.

Effects of exercise at individual anaerobic threshold and maximal fat oxidation intensities on plasma levels of nesfatin-1 and metabolic health biomarkers

Exercise is recognized as an effective method of weight management and short-term appetite regulation tool. The effect of different exercise intensities on appetite regulation hormones in healthy overweight participants has not been intensively studied. The aim of this study was to examine the influence of exercise at individual anaerobic threshold (IAT) and maximal fat oxidation (Fatmax) intensities on the nesfatin-1 response and metabolic health biomarkers in overweight men. Nine healthy overweight males (age, 23.1 ± 1.1 years) volunteered in this study in a counterbalanced order. Blood samples were obtained before, immediately after, and following the first 45 min of recovery for measuring plasma variables. There was significant decrease in plasma levels of nesfatin-1 and leptin after exercise at the IAT intensity which remained lower than baseline following 45 min of recovery. However, nesfatin-1 and leptin levels did not change significantly in any time courses of Fatmax intensity (P > 0.09). Plasma interleukin-6 (IL-6) concentration increased during exercise in both intensities (P < 0.05), whereas changes in free fatty acids (FFAs) and epinephrine concentrations were significant only at the IAT. In addition, a significant correlation was found among nesfatin-1 levels with insulin (r = 0.39, P < 0.05) and glucose (r = 0.41, P < 0.05) at basal and in response to exercise. These results indicate that IAT has a greater exercise-induced appetite regulation effect compared with Fatmax. Based on these data, the intensity of exercise may have an important role in changes of nesfatin-1, leptin, FFA, and epinephrine concentrations even though this was not the case for IL-6 and insulin resistance

Effects of short-term lifestyle activity modification on adiponectin mRNA expression and plasma concentrations.

Adiponectin mRNA and plasma concentrations were reduced in obesity. Exercise training may reduce the adipose tissue (AT), although it is not well known, whether exercise - induced change in AT, increases adiponectin mRNA expression and plasma concentrations or not. Therefore, the purpose of this study was to examine the effects of short-term lifestyle activity modification (LAM) on adiponectin mRNA and plasma concentrations. Sixteen obese and overweight middle-aged men (age, 35-50 years) with type 2 diabetes participated in this study. The subjects were randomly assigned to LAM group (n=8) or control group (n=8). The subjects in LAM group walked two miles in 30 min on a treadmill on 4 days per week for 12 weeks according to the guidelines of the Centers for Disease Control and Prevention and American College of Sports Medicine. The results showed that body mass, body mass index, central visceral adipose tissue and subcutaneous adipose tissue volume and hip and thigh subcutaneous adipose tissue (hip and thigh SAT) volume were decreased in the LAM group compared to the control group (P<0.05). Adiponectin mRNA in abdominal and gluteal subcutaneous AT were increased significantly in the LAM group compared to the control group (P<0.05), while plasma adiponectin concentrations, hs-CRP and insulin resistance did not change significantly. In conclusion, adiponectin mRNA levels increase after 12 weeks of LAM; however, plasma adiponectin levels were not affected by this protocol in obese and overweight middle-aged men with type 2 diabetes.

High-intensity endurance training improves adiponectin mRNA and plasma concentrations.

Adiponectin is an anti-inflammatory protein that reduced in obesity. Exercise training may reduce the adipose tissue (AT), although it is not well known whether exercise-induced change in AT, increases the adiponectin mRNA expression and plasma concentrations or not; therefore, the purpose of this study was to investigate the adiponectin mRNA and plasma concentrations in middle-aged men after 12 weeks high-intensity exercise training and after a week detraining. Sixteen sedentary overweight and obese middle-aged men (age 41.18 ± 6.1 years; ± SD) volunteered to participate in this study. The subjects were randomly assigned to training group (n = 8) or control group (n = 8). The training group performed endurance training 4 days a week for 12 weeks at an intensity corresponding to 75-80% individual maximum oxygen consumption for 45 min. After 12 weeks of training, subjects underwent a week of detraining. The results showed that the BMI as well as central and peripheral AT volume were decreased in the training group compared to the control group (P < 0.05). After 12 weeks, the training group resulted in a significant increase (P < 0.05) in the adiponectin gene expression in abdominal and gluteal subcutaneous AT when compared with the control group. The results showed that plasma adiponectin concentrations increased and insulin resistance decreased after training compared to the control group (P < 0.05). After a week of detraining, the variables were not changed significantly in the training group. In conclusion, high-intensity endurance training caused an increase adiponectin mRNA in obese middle-aged men.

Exercise training intensity/volume affects plasma and tissue adiponectin concentrations in the male rat.

The objective of the study was to determine the effects of exercise training intensity/volume on plasma total and high molecular weight (HMW) adiponectin and tissue total adiponectin concentrations. Thirty-two, eight week-old male Wistar rats (185 ± 5g) were randomly assigned to one of four groups: high intensity (HI: 34 m/min ∼%80-%85 VO(2)max), moderate intensity (MI: 28 m/min ∼%70-%75 VO(2)max), low intensity (LI: 20 m/min ∼ %50-%55 VO(2)max), and sedentary control (SED). Experimental groups completed a 12-week exercise program of treadmill running at 0° slope, 1h/day, 5 days/week. Since frequency and duration of exercise were identical among training groups, the volume of training was highest in the HI group followed by the MI and LI groups. Compared with SED animals, fasting plasma total and HMW adiponectin and adipose tissue total adiponectin concentrations were significantly higher in the HI and MI groups, but total adiponectin concentrations in liver and soleus muscle were not significantly lower than the SED rats. There were significantly lower plasma total testosterone levels in the HI group vs. SED group. Plasma total and HMW adiponectin were negatively correlated with HOMA-IR and insulin whereas total adiponectin was inversely related to TNF-α and HMW adiponectin was negatively correlated with total testosterone. Thus, data suggest there is a dose effect for exercise training intensity and accompanying volume for the adaptation of adipose tissue and circulating total and HMW adiponectin concentrations, whereas the changes of adiponectin concentrations in skeletal muscle and liver tissue may depend on the body's energy balance in the recovery period.